Link:Google Scholar Page
Corresponding authors of each publication are marked with asterisks (*).


  1. Conformational equilibrium of MinE regulates allowable concentration ranges of a protein wave for cell division
    S Kohyama, K Fujiwara*, N Yoshinaga, N Doi
    Nanoscale, in press, doi: 10.1039/D0NR00242A

  2. Self-Organization Assay for Min Proteins of Escherichia coli in Micro-droplets Covered with Lipids
    S Kohyama, K Fujiwara*, N Yoshinaga, N Doi
    bio-protocol, 10(6): e3561, doi: 10.21769/BioProtoc.3561

  3. Microfluidic screening system based on boron-doped diamond electrodes and dielectrophoretic sorting for directed evolution of NAD(P)-dependent oxidoreductases.
    H Goto, Y Kanai, A Yotusui, S Shimokihara, S Shitara, R Oyobiki, K Fujiwara, T Watanabe, Y Einaga, Y Matsumoto, N Miki, N Doi*
    Lab on a chip, 20(4):852-861, doi: 10.1039/C9LC01263J

  4. 2019

    1. Regulation of spatiotemporal patterning in artificial cells by a defined protein expression system
      #A Yoshida, #S Kohyama, #K Fujiwara*, S Nishikawa, N Doi
      (#: contributed equally to this work)
      Chemical Science, 10, pp. 11064-11072 (2019), doi: 10.1039/C9SC02441G

    2. Cell-sized confinement controls generation and stability of a protein wave for spatiotemporal regulation in cells
      S Kohyama, N Yoshinaga*, M Yanagisawa, K Fujiwara*, N Doi
      eLife, doi: 10.7554/eLife.44591
    3. 展望記事

    4. 酵素を内包した人工細胞が拓く新しい醸造業や医薬品の開発
      藤原 慶, MDB 技術予測レポート, 項目36, (2019)

    5. 2018

      1. A dual system using compartmentalized partnered replication for selection of arsenic-responsive transcriptional regulator
        SL Aye, K Fujiwara, N Doi*
        J. Biochem., 164(5), pp. 341-348,

      2. Engineering of DNA polymerase I from Thermus thermophilus using compartmentalized self-replication
        SL Aye, K Fujiwara, A Ueki, N Doi*
        Biochem. Biophys. Res. Comm., 499(2), pp. 170-176

      3. Increasing elasticity through changes in the secondary structure of gelatin by gelation in a microsized lipid space
        A Sakai, #Y Murayama, #K Fujiwara,T Fujisawa, S Sasaki, S Kidoaki, M Yanagisawa*
        (#: contributed equally to this work)
        ACS Central Science, 4(4), pp.477-483

      4. Artificial cell fermentation as a platform for highly efficient cascade conversion
        K Fujiwara*, T Adachi, N Doi
        ACS Synthetic Biology, 7(2), pp 363-370 (2018), doi: 10.1021/acssynbio.7b00365
      5. Review

      6. Single micrometer-sized gels: Unique mechanics and characters for applications
        M Yanagisawa*, C Watanabe, K Fujiwara
        Gels, 4(2), 29, (2018); doi:10.3390/gels4020029
      7. 総説

      8. 細胞サイズ脂質膜小胞による”発酵”生産の可能性
        藤原 慶, バイオサイエンスとインダストリ−, 76(4) pp. 302-303 (2018)

      9. 2017

        1. Liposomal internal viscosity affects the fate of membrane deformation induced by hypertonic treatment
          #K Fujiwara*, #M Yanagisawa*
          (#: contributed equally to this work)
          Soft Matter, 13, pp. 9192-9198 (2017)

        2. Universal glass-forming behavior of in vitro and living cytoplasm
          #K Nishizawa, #K Fujiwara, M Ikenaga, N Nakajo, M Yanagisawa, and D Mizuno*
          (#: contributed equally to this work)
          Scientific Report, 7, Article number: 15143 (2017). doi:10.1038/s41598-017-14883-y

        3. In vitro transcription-translation using bacterial genome as a template to reconstitute intracellular profile
          #K Fujiwara*, #T Sawamura, T Niwa, T Deyama, SM Nomura, H Taguchi, N Doi
          (#: contributed equally to this work)
          Nucleic Acids Research, 45(19), pp. 11449-11458 (2017) DOI: 10.1093/nar/gkx776

        4. DNA cytoskeleton for stabilizing artificial cells.
          C Kurokawa, K Fujiwara, M Morita, I Kawamata, Y Kawagishi, A Sakai, Y Murayama, S M. Nomura, S Murata, M Takinoue*, M Yanagisawa*
          Proc. Natl. Acad. Sci. USA, 114(28), pp. 7228-7233 (2017), doi: 10.1073/pnas.1702208114

        5. Human-derived fusogenic peptides for the intracellular delivery of proteins.
          K Sudo, K Niikura, K Iwaki, S Kohyama, K Fujiwara, N Doi*
          J. Control. Release, 255(10), pp. 1-11 (2017), doi:10.1016/j.jconrel.2017.03.398
        6. 著書

        7. ペプチド医薬品のスクリーニング・安定化・製剤化技術
          技術情報協会, 「1-4. mRNAディスプレイ法によるペプチドのスクリーニング」
          須藤慧, 藤原 慶, 土居信英

        8. 人工細胞の創製とその応用
          CMC出版, 植田充美編,「3-4. 無細胞システムによる生命システムの理解」, 藤原 慶

        9. 2016

          1. In vitro selection of bispecific diabody fragments using covalent bicistronic DNA display.
            M Nakayama, S Komiya, K Fujiwara, K Horisawa, N Doi*
            Biochem. Biophys. Res. Comm., 478(16), pp. 606-611 (2016)

          2. Biochemical preparation of cell extract for cell-free protein synthesis without physical disruption.
            K Fujiwara*, N Doi
            PLOS ONE, 11(4): e0154614. (2016) doi:10.1371/journal.pone.0154614

          3. Identification of novel in vivo obligate GroEL/ES substrates based on data from a cell-free proteomics approach.
            T Niwa, K Fujiwara, H Taguchi*
            FEBS Lett., 590 (2), pp. 251-257 (2016)
          4. 翻訳

          5. 自然世界の高分子 (吉岡書店, 原題:Giant Molecules)
            原著:Alexander Y. Grosberg and Alexei R. Khokhlov、監訳: 田中 基彦、鴇田 正之
            訳: 田中 基彦、鴇田 正之、柳沢 実穂、坂上 貴洋、藤原 慶
          6. 著書

          7. DNA分子デザインのすべて〜BIOMOD虎の巻
            CBT学会eBOOK、分子ロボティクス研究会編(1章3,4,5,11の執筆担当:藤原 慶)

          8. 2015

            1. PURE mRNA display for in vitro selection of single-chain antibodies.
              Y Nagumo, K Fujiwara, K Horisawa, H Yanagawa, N Doi*
              J. Biochem., 159(5), pp. 519-526 (2015)

            2. Droplet-Shooting and Size-Filtration (DSSF) Method for Synthesis of Cell-Sized Liposomes with Controlled Lipid Compositions.
              M Morita, H Onoe, M Yanagisawa, H Ito, M Ichikawa, K Fujiwara, H Saito, M Takinoue*
              ChemBioChem, 16 (14), pp. 2029-2035 (2015), DOI: 10.1002/cbic.201500354

            3. Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device.
              H Yamashita, M Morita, H Sugiura, K Fujiwara, H Onoe, M Takinoue*
              J. Biosci. Bioeng., 119 (4), pp. 492-495 (2015), DOI: 10.1016/j.jbiosc.2014.09.018

            4. Peptide sequences converting polyglutamine into a prion in yeast.
              W Odani, K Urata, M Okuda, S Okuma, H Koyama, CG Pack, K Fujiwara, T Nojima, M Kinjo, S Kawai-Noma, H Taguchi*
              FEBS Lett., 282 (3), pp. 477-490 (2015)
            5. 総説

            6. 高分子混雑効果を細胞モデル系から読み解く
              柳澤 実穂, 藤原 慶
              生物物理, 55(5) pp. 246-249 (2015)


            1. Multiple patterns of polymer gels in microspheres due to the interplay among phase separation, wetting, and gelation.
              M Yanagisawa*, S Nigorikawa, T Sakaue, K Fujiwara, M Tokita.
              Proc. Natl. Acad. Sci. USA, 111 (45), 15894-15899 (2014)

            2. Conversion of a Chaperonin GroEL-Independent Protein into an Obligate Substrate.
              T Ishimoto, K Fujiwara, T Niwa, H Taguchi*.
              J. Biol. Chem., 289: 32073-32080 (2014)

            3. Introducing Micrometer-Sized Artificial Objects into Live Cells: A Method for Cell-Giant Unilamellar Vesicle Electrofusion.
              AC Saito, T Ogura, K Fujiwara, S Murata, SM Nomura*.
              PLoS ONE, (9), e106853 (2014)

            4. Generation of Giant Unilamellar Liposomes Containing Biomacromolecules at Physiological Intracellular Concentrations using Hypertonic Conditions.
              K Fujiwara*, M Yanagisawa*.
              ACS Synthetic Biology, 3 (12), pp 870-874 (2014): 10.1021/sb4001917
            5. Review

            6. Reconstitution of intracellular environments in vitro and in artificial cells.
              K Fujiwara*, M Yanagisawa, SM Nomura.
              BIOPHYSICS, 10 (0), pp. 43-48 (2014)
            7. 和文

            8. DNAナノテクノロジーのためのアウトリーチツールの開発と評価.
              吉澤 慧, 藤原 慶, 野村 M.慎一郎, 村田 智*
              形の科学会誌, 2014, 29(2): 表紙を飾りました
            9. 紀要

            10. The rapid synthesis of cell-sized liposomes by centrifuge-based microfluidic device.
              M Morita, H Onoe, M Yanagisawa, K Fujiwara, H Saito, M Takinoue.
              18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, pp. 336-338 (2014)
            11. 著書

            12. これだけ! 生化学 (秀和システム)
              著:生化学若い研究者の会 (執筆、編集を分担)、監修:稲垣賢二


            1. A bacterial salt sensor created by multiplying phenotypes of GroE-depleted Escherichia coli.
              K Fujiwara*, KB Aoi, SM Nomura*.
              Analytical methods, 5 (21), 5918-5922 (2013)

            2. Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system.
              K Fujiwara*, T Katayama, SM Nomura.
              Nucleic Acids Research, 41 (14): 7176-7183 (2013) doi:10.1093/nar/gkt489

            3. Condensation of an additive-free cell extract to mimic the conditions of live cells.
              K Fujiwara*, SM Nomura*.
              PLoS ONE 8(1): e54155 (2013) doi:10.1371/journal.pone.0054155

            4. Physicochemical analysis from real-time imaging of liposome tubulation reveals the characteristic of individual F-BAR domain proteins.
              Y Takiguchi, T Itoh, K Tsujita, S Yamada, M Yanagisawa, K Fujiwara, M Ichikawa, A Yamamoto, K Takiguchi*.
              Langmuir, Jan 8;29(1):328-36 (2013)
            5. 紀要

            6. Molecular robotics approach for constructing artificial cell model
              SM Nomura*, Y Sato, K Fujiwara.
              12th European Conference on Artificial Life, ECAL, 488-491 (2013)

            7. Construction of remote-control supramolecular micro-crawler
              D Komatsu, K Fujiwara, SM Nomura*.
              12th European Conference on Artificial Life, ECAL, 208-209 (2013)
            8. 総説

            9. 細胞内濃厚環境に近づいた無細胞発現系
              藤原 慶, 野村 M. 慎一郎, 生物物理, 53(5), pp.262-263 (2013)

            10. 細胞抽出液の濃縮技術〜細胞再構築に向けた挑戦〜
              野村 M. 慎一郎, 藤原 慶, 月刊バイオインダストリ, Vol. 30、No.8, p.59-65 (2013)


            1. Cell-Sized Confinement in Microspheres Accelerate the Reaction of Gene Expression.
              A Kato, M Yanagisawa, YT Sato, K Fujiwara, K Yoshikawa*.
              Scientific Report, 2, Article number: 283 (2012)

            2. Mechanism of overexpression of methionine synthase in chaperonin-depleted Escherichia coli.
              K Fujiwara*, H Taguchi.
              Microbiology, 158, 917-924 (2012)


            1. A systematic survey of in vivo obligate chaperonin-dependent substrates.
              K Fujiwara, Y Ishihama, K Nakahigashi, T Soga, H Taguchi*.
              The EMBO Journal, Vol. 29, No.9, 1552-1564 (2010)

            2. Filamentous morphology in GroE-depleted Escherichia coli induced by impaired folding of FtsE.
              K Fujiwara, H Taguchi*.
              J. Bacteriol., Vol. 189, No. 16, 5860-5966 (2007)

            3. Involvement of the arginine repressor in lysine biosynthesis of Thermus thermophilus.
              K Fujiwara, T Tsubouchi, T Kuzuyama, Nishiyama M*.
              Microbiology, vol. 152, 3585-3594 (2006)

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